The effort to understand the genome and all of its components at a functional level will impact drug development and basic discovery in powerful ways, and a fundamental part of this effort is technology. Tools enabled by the sequence of the genome and parallel advances in automation, high throughput screening, proteomics, cell biology, chemical/genetic libraries, imaging, and computation now allow researchers to query basic biological phenomena in an unprecedented manner.
At GNF, these technologies have been brought together under one roof and are employed in a multidisciplinary fashion to understand the genome and all its components at a functional level. Our efforts fall into multiple categories, including:
- Discovering novel genes, proteins and druggable targets involved in signaling pathways using high throughput screening (HTS) coupled with small molecule and/or siRNA/cDNA library screens in cell-based phenotypic assays
- Developing high throughput automated systems and incorporating high content imaging principles to generate and screen chemical and gene-based libraries
- Creating methods for mining data from large representative panels of small molecule and genetic libraries to find functional clustering, potential targets, and mechanisms of action
- Implementing automated compound profiling systems to functionally characterize small collections of compounds, such as known drugs or families of genes, in an effort to predict a range of factors from pathway physiology to toxicity. Our core capabilities in automation, screening, library development, imaging, and computation form the basis of the Cellular Technologies effort at GNF.